Protection of Bovine Mammary Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Cell Damage by Resveratrol

Figure 1

Protective effects of resveratrol against H₂O₂-induced MAC-T cell death and ROS production. (a) MAC-T cells were treated with increasing concentrations of H₂O₂ (0, 250, 500, and 1000 µM) for 12 h or 24 h. Cell viability was measured by CCK-8 assay (left panel), and ROS concentration was measured by flow cytometer after being loaded with 10 µM DCFH-DA fluorescence probe for 30 min (right panel). , , and significantly different from untreated cells. (b) MAC-T cells were pretreated with the indicated concentrations of resveratrol for 2 h, followed by H₂O₂ (500 μM) challenge for 24 h. Then the cell viability (black bars) and ROS concentration (grey bars) were determined (left panel). A representative flow cytometric histogram is shown (right panel). (c) MAC-T cells were pretreated with or without 50 μM of resveratrol for 2 h and then treated with or without 500 μM of H₂O₂. The mRNA expression of the endoplasmic reticulum stress markers GRP 78 and CHOP (4 hours after H₂O₂ treatment) and mitochondria-related cell apoptosis markers Bax and Bcl-2 (24 h after H₂O₂ treatment) was analyzed by quantitative real-time PCR. Data are represented as mean ± SD from three independent experiments. means significantly different from untreated cells. means significantly different from H₂O₂-treated cells.

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