Protection of Bovine Mammary Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Cell Damage by Resveratrol

Figure 5

ERK and Akt pathway activations mediate cytoprotective effects of resveratrol against oxidative stress. MAC-T cells were preincubated with or without specific kinase inhibitors LY294002 (25 μM), PD98059 (100 μM), SP600125 (10 μM), or SB203580 (25 μM) for 1 h and then treated with or without resveratrol for another 2 h, followed by H₂O₂ exposure. mRNA expression of HO-1, TrxR-1, xCT, and Nrf2 was measured 8 h after H₂O₂ stimulation (a) and cell viability and ROS levels were analyzed 12 h after H₂O₂ stimulation (b). Values are the means ± SD from three experiments, , , and .

(a)

(b)