Research Article

Protection of Bovine Mammary Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Cell Damage by Resveratrol

Figure 6

Schematic diagram summarizing the mechanisms underlying the protective effect of resveratrol against H2O2-induced oxidative stress in MAC-T cells (detailed in Section 4). H2O2 mediated oxidative stress in MAC-T cells and enhanced the accumulation of ROS. ROS also mediated the upregulations of mitochondria-mediated cell apoptosis genes and ER stress genes, leading to cell death. Resveratrol reduced ROS production by H2O2 and induced expressions of several antioxidant-stress response genes (HO-1, Txnrd-1, and xCT) at Nrf2 dependent manner. Nrf2-related antioxidant-stress response genes induction by resveratrol during H2O2 mediated oxidative stress involved stress response protein kinases (mainly PI3K/Akt and ERK/MAPK) but was negatively regulated by p38 MAPK.