(Z)-5-(2,4-Dihydroxybenzylidene)thiazolidine-2,4-dione Prevents UVB-Induced Melanogenesis and Wrinkle Formation through Suppressing Oxidative Stress in HRM-2 Hairless Mice

Figure 2

MHY498 ameliorates UVB-induced collagen fiber destruction. MHY498 was pretreated for 3 days. From day 4, 2 h after MHY498 application to the dorsal skin of the hairless mice, UVB was exposed to the mice every other day for 1 h. After 4 weeks, skin samples were excised and Masson’s trichrome staining was performed as described in Materials and Methods (a–d). Control mice without UVB exposure (a), mice with UVB exposure (b), and MHY498 (0.5 μM) (c) or MHY498 (5 μM) treated mice (d). Collagen staining appears blue. Collagen area was calculated based on microscopic analysis of the dorsal skin staining (/group) (e). Data are represented as mean ± SEM. compared to a control group without UVB exposure and compared to the group with UVB exposure.

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