(Z)-5-(2,4-Dihydroxybenzylidene)thiazolidine-2,4-dione Prevents UVB-Induced Melanogenesis and Wrinkle Formation through Suppressing Oxidative Stress in HRM-2 Hairless Mice

Figure 4

MHY498 reduces protein levels of MMPs and procollagens. MHY498 was pretreated for 3 days. From day 4, 2 h after MHY498 application to the dorsal skin of the hairless mice, UVB was exposed to the mice every other day for 1 h. After 4 weeks, skin samples were excised and homogenated. Western blotting was performed to detect protein levels of MMP1, MMP9, MMP12, MMP13, and β-actin (/group) (a). Protein levels of type 1 and type 3 procollagen and β-actin (/group) (b). For western blotting results, a representative blot is shown from four experiments that yielded similar results. Hs27 human dermal fibroblasts were cultured in DMEM until reaching 85% confluence. Cultured human fibroblasts were pretreated with MHY498 for 2 h and exposed to UVB (30 mJ/cm²). Type 1 procollagen concentration was measured using a commercially available ELISA kit (/group) (c). Data are represented as mean ± SEM. compared to a control group without UVB exposure and compared to the group with UVB exposure.

(a)

(b)

(c)