(Z)-5-(2,4-Dihydroxybenzylidene)thiazolidine-2,4-dione Prevents UVB-Induced Melanogenesis and Wrinkle Formation through Suppressing Oxidative Stress in HRM-2 Hairless Mice

Figure 5

MHY498 reduces UVB-induced oxidative stress and related signaling. MHY498 was pretreated for 3 days. From day 4, 2 h after MHY498 application to the dorsal skin of the hairless mice, UVB was exposed to the mice every other day for 1 h (/group). After 4 weeks, skin samples were excised and ROS (a) and ONOO⁻ (b) generation were measured using DCF-DA and DHR-123 as fluorescent probes (/group). (c) Western blotting images of p-p65 (S536), p65, and TFIIB in the nucleus extracts. (d) Protein levels of NF-κB target gene iNOS in the cytosol extracts. For western blotting results, a representative blot is shown from four experiments that yielded similar results. NF-κB, nuclear factor-κB; TFIIB, transcription factor II B. Each value was expressed as mean ± SEM. and versus control without UVB exposure; and versus the UVB-exposed group.

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