Pomegranate-Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3-Mediated SOD2 Activation

Figure 2

Effects of SIRT3-augmenting polyphenols on SOD2 activity. Caco-2 cells were treated with SIRT3-augmenting polyphenols (10 μM) for 48 h and SOD2 activity was measured using the SOD Assay Kit-WST (a). Deacetylation of SOD2 by pomegraniin A; Caco-2 cells were transduced with Flag-SOD2 and treated with 10 μM of eucalbanin B or pomegraniin A for 48 h. Flag-SOD2 was immunoprecipitated with anti-Flag M2 antibodies immobilized to Protein G and analyzed by immunoblot using anti-acetylated-lysine antibodies (b and c). Band intensity corresponding to acetylated SOD2 was quantitatively determined using Image J software. The results are expressed as mean ± standard deviation. Control Caco-2 cells were treated with the same volume of DMSO used for treating with 10 μM of polyphenols. Statistical significance between the respective polyphenol and control was determined by one-way ANOVA with Tukey’s post hoc test. Statistical significance was defined as (; ).

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