Pomegranate-Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3-Mediated SOD2 Activation

Figure 4

Pomegraniin A reduces intracellular ROS levels via SIRT3. Effects of pomegraniin A on intracellular ROS levels in Caco-2 cells; cells were treated with 10 μM pomegraniin A for 48 h or 10 mM NAC for 2 h prior to detection, and intracellular ROS levels were analyzed with an IN Cell Analyzer 1000. The relative number of the cells with high ROS levels was shown in the scatter plot (a). Effects of pomegraniin A on intracellular ROS levels in SIRT3-silenced Caco-2; cells were treated with 10 μM pomegraniin A for 48 h, followed by detection of ROS levels with an IN Cell Analyzer 1000. The relative number of the cells with high ROS levels was shown in the scatter plot (b). Control Caco-2 cells were treated with the same volume of DMSO used for treating with 10 μM of polyphenols. Statistical significance between samples and control was determined by one-way ANOVA with Tukey’s post hoc test. Statistical significance was defined as   (). NAC, N-acetyl cysteine; ROS, reactive oxygen species; DMSO, dimethyl sulfoxide. Experiments were repeated three times, and representative was shown.

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(b)