Modulation of Tamoxifen Cytotoxicity by Caffeic Acid Phenethyl Ester in MCF-7 Breast Cancer Cells

Figure 1

Survival fraction in MCF-7 cells after 48 h treatment with TAM, CAPE, and their combination. Cells were treated with various concentrations of (a) TAM, (b) CAPE, (c) TAM + CAPE, and (d) 10 μM TAM + CAPE for 48 h. (e) Isobologram analysis of MCF-7 cell growth inhibition by TAM and CAPE after 48 h of treatment. The IC₅₀ value of each drug after 48 h is plotted on the axes; the solid line represents the additive effect, while the asterisk () located below the connecting line points to the synergistic effect of the concentrations of TAM and CAPE used in the combination. Results were expressed as means ± SD of 3 independent experiments performed in triplets. value < 0.05 is considered significant. A: significantly different from the respective concentration of control. B or $: significantly different from the respective concentration of TAM. ◆: significantly different from the respective concentration of CAPE.

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