Research Article

2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside Promotes Expression of the Longevity Gene Klotho

Table 1

Primer sequences of target genes and annealing temperature.

GenePrimer sequencesAnnealing temperature

Klotho (138 bp)Sense: 5′-ACTTTCTTCTGCCCTATTTCACG-3′58°C
Antisense: 5′-CCAGGTAATCGTTGTATTTTATCGG-3′
GAPDH (192 bp)Sense: 5′-GATCCCGCTAACATCAAATG-3′58°C
Antisense: 5′-GAGGGAGTTGTCATATTTCTC-3′