Review Article

DNA Damage in Chronic Kidney Disease: Evaluation of Clinical Biomarkers

Table 3

Outcome of studies comparing 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) levels of healthy individuals and patients on maintenance hemodialysis3.

8-oxodG measured inMethodNumber of healthy individuals (age)Number of dialyzed patients (age)Relative change (%)Mean ± stddevRef.

DNA of PBLsHPLC-ECD35 (60 ± ?)109 ()+160[47]
DNA isolated from bloodHPLC-ECD9 ()29 ()+180[42]
DNA isolated from bloodHPLC-ECD55 ()44 ()+275[48]
Nuclear DNA from mononuclear cellsHPLC-ECD67 ()30 ()+125[49]

Mitochondrial DNA from mononuclear cellsHPLC-ECD67 ()30 ()+11[49]

SerumELISA9 (?)73 ()+700[50]
SerumELISA16 (?)71 (?)+155[51]
SerumELISA10 ()9 ()+55[52]

The number of participants in the respective studies, their age, and the amount of relative change in the parameter in percent and rounded are given. From these, the mean and the standard deviation (stddev) were calculated. When more than one sample was taken from the dialysis patients, the value of the predialytic sample was compared to the control value. In the last column the reference (Ref.) is listed. PBLs: peripheral blood lymphocytes. HPLC-ECD: high performance liquid chromatography with electrochemical detection. ?: no standard error mean or no age at all was given.