Research Article

Macroautophagy and Selective Mitophagy Ameliorate Chondrogenic Differentiation Potential in Adipose Stem Cells of Equine Metabolic Syndrome: New Findings in the Field of Progenitor Cells Differentiation

Figure 1

Characterization of isolated ASC analysis of surface antigens and multipotency assay. Using flow cytometer, the expression of CD44, CD45, CD90, and CD105 antigens was investigated. Isolated cells were characterized by the expression of CD44, CD90, and CD105 while they lacked the expression of CD45 hematopoietic marker (a). Interestingly, ASCEMS displayed higher expression of CD44. Multipotency of ASC was confirmed by trilineage differentiation assay. Representative photographs showing the effectiveness of chondrogenesis (Safranin O), osteogenesis (Alizarin Red), and adipogenesis (LipidTox) (b). Cells cultured in standard culture medium served as a control.
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