PD98059 Protects Brain against Cells Death Resulting from ROS/ERK Activation in a Cardiac Arrest Rat Model

Figure 4

Effects of PD98059 (PD) on the expression of caspase-3. (a) Representative western blot of cleaved caspase-3 in brain of rat subjected to CA/CPR at 12, 24, 48, and 72 h following reperfusion. (b) Cleaved caspase-3 band intensity normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Representative western blot showing the cleaved caspase-3 significantly increased in CA group and PD at 12 h after CA/PCR compared to sham operation (). The increase of cleaved caspase-3 was sustained to 72 h of reperfusion in CA. In contrast, treatment with PD98059 decreased significantly caspase-3 from 24 h to 72 h; mean ± standard deviation. for each group (A = versus the sham group; B = versus the CA group).

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