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Oxidative Medicine and Cellular Longevity
Volume 2016, Article ID 3859721, 8 pages
Research Article

Phlorizin, an Active Ingredient of Eleutherococcus senticosus, Increases Proliferative Potential of Keratinocytes with Inhibition of MiR135b and Increased Expression of Type IV Collagen

1Department of Dermatology, Seoul National University Bundang Hospital, Seongnam, Gyeonggi 463-707, Republic of Korea
2Biospectrum Life Science Institute, Seongnam, Gyeonggi 462-807, Republic of Korea
3Department of Genetic Engineering, Sungkyunkwan University, 2066 Seobu-ro, Suwon City, Gyunggi Do 164-19, Republic of Korea
4Department of Medical Pharmacy, Hyogo University of Health Sciences, Kobe 650-8530, Japan
5P&G Group, Kobe 658-0032, Japan

Received 8 October 2015; Revised 15 January 2016; Accepted 17 January 2016

Academic Editor: Denis Delic

Copyright © 2016 Hye-Ryung Choi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


E. senticosus extract (ESE), known as antioxidant, has diverse pharmacologic effects. It is also used as an antiaging agent for the skin and phlorizin (PZ) is identified as a main ingredient. In this study, the effects of PZ on epidermal stem cells were investigated. Cultured normal human keratinocytes and skin equivalents are used to test whether PZ affects proliferative potential of keratinocytes and how it regulates these effects. Skin equivalents (SEs) were treated with ESE and the results showed that the epidermis became slightly thickened on addition of 0.002% ESE. The staining intensity of p63 as well as proliferating cell nuclear antigen (PCNA) is increased, and integrin α6 was upregulated. Analysis of ESE confirmed that PZ is the main ingredient. When SEs were treated with PZ, similar findings were observed. In particular, the expression of integrin α6, integrin β1, and type IV collagen was increased. Levels of mRNA for type IV collagen were increased and levels of miR135b were downregulated. All these findings suggested that PZ can affect the proliferative potential of epidermal cells in part by microenvironment changes via miR135b downregulation and following increased expression of type IV collagen.