The Relevance of Nrf2 Pathway and Autophagy in Pancreatic Cancer Cells upon Stimulation of Reactive Oxygen Species
Silencing of Nrf2 promotes autophagy upon ROS stimulation in PANC-1 cells. (a) Western blot detection of Nrf2 in siRNA-transfected PANC-1 cells. (b) The expression of Beclin1 and LC3-II and also the level of p62 in Nrf2 siRNA-transfected PANC-1 cells upon ROS stimulation. (c-d) Immunofluorescence images of PANC-1 cells for Beclin1 and LC3 upon ROS stimulation. (e) Numbers of autophagosomes were counted in 10 random fields. The data are presented as Mean ± SD for three independent experiments. Column: Mean; bar: SD.