Hyperglycemia Aggravates Hepatic Ischemia Reperfusion Injury by Inducing Chronic Oxidative Stress and Inflammation

Figure 2

Hepatic oxidative stress and inflammation in normal or diabetic rats. (a) Nuclear 8-OHdG (400x) detected by IHC staining and IOD assessed by Image-J. (b) Serum H₂O₂ levels. (c) Hepatic MDA levels. (d) Hepatic 8-isoprostane determined by ELISA. (e) Hepatic inflammatory cytokines determined by ELISA. Data are expressed as mean ± SEM ( per group). versus normal sham group; versus normal sham group; versus normal sham group; versus diabetic sham group; versus diabetic sham group; versus diabetic sham group; versus normal HIRI group; versus normal HIRI group; versus normal HIRI group. HIRI: hepatic ischemia reperfusion injury; 8-OHdG: 8-hydroxydeoxyguanosine; IOD: integrated optical intensity; IHC: immunohistochemistry; H₂O₂: hydrogen peroxide; MDA: malondialdehyde; TNF-α: tumor necrosis factor α; MCP-1: monocyte chemokine protein-1; IL-6: interleukin-6; ELISA: enzyme-linked immunosorbent assay; sham: sham operating group.

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