Research Article

Coordinated Upregulation of Mitochondrial Biogenesis and Autophagy in Breast Cancer Cells: The Role of Dynamin Related Protein-1 and Implication for Breast Cancer Treatment

Figure 1

Analysis of mitochondrial dynamics regulators. (a) Gene expression of DNM1L (encoding the mitophagy activator Drp1) in normal breast tissues () and breast cancer (BRCA, ) tissues. The data were extracted from TCGA (The Cancer Genome Atlas) database at Insilicom (http://insilicom.com/). (b)-(c) Immunohistochemistry analysis of Drp1 in normal breast (b) and invasive carcinoma (c) tissues. Scale bar: 100 μm; arrows indicate massively positive staining of Drp1 in the breast cancer tissues. (d) Western blot analysis of mitochondrial fission (Drp1) and fusion (Mfn1 and Mfn2) proteins in MCF-10A (abbreviated as “10A”) and MDA-MB-231 (abbreviated as “231”) cells. (e)-(f) Densitometric analysis of western blot images as shown in (d) (–5). ; ; n.s.: not significant.
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