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Figure 5: Effects of Drp1 inhibitor Mdivi-1 on mitochondrial autophagy, metabolism, and viability of MDA-MB-231 cancer cells. (a)-(b) Mdivi-1 suppressed mitophagy marker BNIP3 and caused accumulation of LC3-II, indicative of reduced autophagic flux. MDA-MB-231 cells were cultured and treated with Mdivi-1 as described in the Materials and Methods, and the protein levels were analyzed by western blot (a) and densitometric (b) analysis; –5. (c)-(d) Mdivi-1 promoted mitochondrial oxidative capacity, as evaluated by respiratory control ratios (RCR) and ATP turnover. (e) Mdivi-1 suppressed glycolysis (). (f) Mdivi-1 reduced breast cancer cell viability (–7). and .