Phenolic Rich Extract from Clinacanthus nutans Attenuates Hyperlipidemia-Associated Oxidative Stress in Rats

Table 1

Gene name, accession number, and sequences of primers used in multiplex panel analysis.

Gene name		Primer sequence (with universal tag)
		Forward primer	Reverse primer
CAT	NM_012520	AGGTGACACTATAGAATACATTCTATACGAAGGTGTTG	GTACGACTCACTATAGGGAGGTGTGAATTGCATTCTTAG
SOD1	NM_017050	AGGTGACACTATAGAATATCAATATGGGGACAATACAC	GTACGACTCACTATAGGGATACTTTCTTCATTTCCACCTT
SOD2	NM_017051	AGGTGACACTATAGAATAACTTTGGGTCTTTTGAGAA	GTACGACTCACTATAGGGATTCACTTCTTGCAAACTATG
GPx1	NM_030826	AGGTGACACTATAGAATAGGCAAGAATGAAGAGATTC	GTACGACTCACTATAGGGACTACCAGGAACTTCTCAAAG
GSR	NM_053906	AGGTGACACTATAGAATAAGCCTGGGGATAACCAGTGA	GTACGACTCACTATAGGGAAATGTAACCGGCACCCACAA
PPIA	NM_017101	AGGTGACACTATAGAATATTCTGTAGCTCAGGAGAGCA	GTACGACTCACTATAGGGATTGAAGGGGAATGAGGAAAA
GAPDH	NM_017008	AGGTGACACTATAGAATAATGACTCTACCCACGGCAAG	GTACGACTCACTATAGGGAAGCATCACCCCATTTGATGT
KanR

Housekeeping gene. Internal control. Normalization gene. Reverse transcription (RT) and PCR were done according to manufacturer’s instructions; RT reaction was at 48°C for 1 min; 37°C for 5 min; 42°C for 60 min; 95°C for 5 min and then held at 4°C, while PCR was as follows: initial denaturation at 95°C for 10 min, followed by two-step cycles of 94°C for 30 sec and 55°C for 30 sec, ending in a single-extension cycle of 68°C for 1 min. CAT: catalase; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; GPx: glutathione peroxidase; GSR: glutathione reductase; KanR: kanamycin resistant; PPIA: cyclophilin A; SOD: superoxide dismutase.