SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 Cells

Figure 5

SOD2-siRNA reversed amifostine-induced effects on apoptosis. The PC12 cells were divided into six groups: control: cells cultured in drug-free medium; Ami: cells exposed to 500 μM amifostine for 24 h; Glu: cells treated with 15 mM glutamate for 24 h; Ami + Glu: cells exposed to 500 μM amifostine plus 15 mM glutamate for 24 h; SOD2-siRNA + Ami + Glu: cells incubated with SOD2-siRNA transfection complexes for 24 h and then exposed to 500 μM amifostine plus 15 mM glutamate for 24 h; SC-siRNA + Ami + Glu: cells incubated with scrambled- (SC-) siRNA transfection complexes for 24 h and then exposed to 500 μM amifostine plus 15 mM glutamate for 24 h. Apoptotic rate was determined by a flow cytometry, and the expression of cleaved (CLV) caspase-3 was evaluated by western blot analysis. Results are means ± SD, , and NS: no significance. (a) Apoptotic rates assessed by flow cytometry. (b) Statistical results of apoptotic rates. (c) SOD2-siRNA abolished amifostine-induced decrease of cleaved caspase-3 expression. Results are means ± SD, , and NS: no significance.

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