PTEN Activation by DNA Damage Induces Protective Autophagy in Response to Cucurbitacin B in Hepatocellular Carcinoma Cells

Figure 5

Cuc B induced ROS formation. Cells were treated with Cuc B for 1 h and the ROS generation was detected with DCFH₂-DA (a). Cells were treated with Cuc B (100 nM) and the ROS formation was detected (b). Cells were pretreated with NAC (2.5 mM) for 1 h followed by cotreatment with Cuc B for 6 h and the ROS (c) and cell viability were determined (d). H₂O₂ (1 mM), positive control. Cuc B, Cucurbitacin B; ROS, reactive oxygen species. ; ; .

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