Research Article

Monoamine Oxidase Is Overactivated in Left and Right Ventricles from Ischemic Hearts: An Intriguing Therapeutic Target

Figure 4

Oxidative stress markers. MDA (a) and protein carbonyls (b) content in RV and LV of NF and failing IHD ventricles. Data are expressed as mean ± SEM. failing versus NF LV; failing versus NF RV. Actin carbonylation was obtained subjecting derivatized cardiac proteins to SDS-PAGE and immunoblot with anti-DNP antibodies (Oxyblot) (c) followed by stripping and reprobing with anti-actin antibodies (d). Actin oxidation index (e) is given by the ratio between the densitometric values of the bands in the Oxyblot and those of the corresponding bands in the anti-actin immunoblot; failing versus NF. NF; IHD.
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