Research Article

Apigenin Attenuates Oxidative Injury in ARPE-19 Cells thorough Activation of Nrf2 Pathway

Figure 1

Apigenin protects against t-BHP-induced oxidative injury in ARPE-19 cells. (a, b) MTT assay for determining cell inhibition rate and cell survival rate when exposed to t-BHP. Significance: versus control; versus control. (c) MTT assay for determining whether apigenin had toxic effects on cells. (d) MTT assay for determining the protective effects of apigenin on t-BHP-treated cells. Significance: versus control; versus t-BHP. (e) Flow cytometry analyses of apoptosis using FITC-labeled Annexin V/PI staining. Significance: versus control; versus t-BHP. (f) Representative charts of flow cytometry analyses of apoptosis. Cells situated in the right two quadrants of each plot were regarded as apoptotic cells.
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