Acetaldehyde Induces Cytotoxicity of SH-SY5Y Cells via Inhibition of Akt Activation and Induction of Oxidative Stress

Figure 4

Acetaldehyde increases oxidative stress in SH-SY5Y cells. (a) SH-SY5Y cells were treated with 10 mM of acetaldehyde for 1 h. Cells were then stained with DCFH-DA to determine the production of ROS. Images on the left side are the phase images of the cells. (b) SH-SY5Y cells were treated with 5 mM of acetaldehyde for 2 h. The levels of reduced GSH were determined in the cell lysates. (c) SH-SY5Y cells were treated with different concentration of acetaldehyde for 24 h. MDA production was measured in the cell lysates. Values are means ± SD. , significantly different from control cells (); , significantly different from control cells ().

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