The C-ETS2-TFEB Axis Promotes Neuron Survival under Oxidative Stress by Regulating Lysosome Activity

Figure 1

Lysosome functions are critical for cell survival under oxidative stress. (a) Primary neurons were treated with H₂O, H₂O₂ (50 μM), or etoposide (80 μg/mL) as a positive control for 4 h, and cell apoptosis was detected by flow cytometry. (b) Primary neurons were treated with different concentrations of hydrogen peroxide or etoposide for 4 h, and cell apoptosis was detected by immunoblotting with PARP and caspase-3 antibodies. (c, d, e, f) Primary neurons were pretreated with the lysosome inhibitor bafilomycin A1, chloroquine, or ammonium chloride for 1 h and then exposed to H₂O₂ (150 μM) for 4 h. The degree of apoptosis was analysed by western blotting or flow cytometry. The flow cytometry data were quantified, and the results are shown in (f). Values are the means ± SEM. compared with the control group.

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