The C-ETS2-TFEB Axis Promotes Neuron Survival under Oxidative Stress by Regulating Lysosome Activity

Figure 3

The increased expression of lysosomal genes is regulated by TFEB under oxidative stress. (a) 293T cells were transfected with PGE1-shTFEB plasmids or PGE1 empty plasmids as a control and, after 24 h, were treated with H₂O₂ for 4 h. Lysosomal genes were analysed by RT-PCR. Values are the means ± SEM. , compared with the empty vector group. (b) SHY5Y cells were treated with H₂O₂ or etoposide, and the protein level of TFEB was detected. (c) Primary neurons were treated with H₂O₂, and the protein level of TFEB was detected. (d) The mRNA level of TFEB in SHY5Y cells, primary neurons, and mouse brains was measured using RT-PCR. Values are the means ± SEM. , compared with the control group, and compared with the MPTP group. (e) 293T cells were transfected with PGL3 constructs containing the TFEB promoter and the Renilla plasmid, and a luciferase assay was performed to measure the transcription activity of TFEB in the presence of H₂O₂ or etoposide. Values are the means ± SEM. compared with the H₂O group.

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