Equine Metabolic Syndrome Affects Viability, Senescence, and Stress Factors of Equine Adipose-Derived Mesenchymal Stromal Stem Cells: New Insight into EqASCs Isolated from EMS Horses in the Context of Their Aging

Figure 1

Immunophenotyping of EqASCs. Representative photographs showing the results of EqASC immunophenotyping. (a) The cells expressed the following mesenchymal markers CD44, CD73, CD90, and CD105 and showed no expression of hematopoietic marker CD45. Markers were detected with specific antibodies and visualized with secondary antibodies conjugated with atto-488; nuclei were counterstained with DAPI. Magnification ×100, scale bar: 250 μm. The presence of antigens CD44 (b), CD73 (c), CD90 (d), and CD105 (e) was compared using the relative fluorescent intensity. Results expressed as mean ± SD. value < 0.05; value < 0.001.

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