Equine Metabolic Syndrome Affects Viability, Senescence, and Stress Factors of Equine Adipose-Derived Mesenchymal Stromal Stem Cells: New Insight into EqASCs Isolated from EMS Horses in the Context of Their Aging

Figure 6

Evaluation of apoptosis and senescence in and . Assessment of apoptosis and senescence (a). Pictures showing the results of calcein: live cells (i, iv); propidium iodide: dead cells (ii, v); and β-galactosidase: senescence cells (iii, vi) staining. Percentage of dead cells in the cultures was evaluated based on the calcein/propidium iodide staining (b). Differences in the accumulation of β-galactosidase were determined based on the percentage of stained area (c). lc: live cell, dc: dead cell, and β-gal: β-galactosidase. Magnification ×100, scale bar: 250 μm. Results expressed as mean ± SD. value < 0.05.

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