Equine Metabolic Syndrome Affects Viability, Senescence, and Stress Factors of Equine Adipose-Derived Mesenchymal Stromal Stem Cells: New Insight into EqASCs Isolated from EMS Horses in the Context of Their Aging

Figure 8

Mitochondria and reactive oxygen species in EqASCs. Images showing DAPI (i, vi), Mito Red (ii, vii), and ROS staining (iii, viii). Magnification ×100, scale bar: 250 μm. Analysis of mitochondrial morphology by a transmission electron microscope (iv, v, ix, x). Representative electron micrographs showing alternations in mitochondrial morphology in . Small structures with disarrayed cristae are indicated with black arrows; membrane raptures and vacuole formation are indicated with red arrows. Quantitative assessment of ROS by the relative fluorescent intensity (b). Percentage of abnormal mitochondria (c); the width (d); and length (e) of mitochondria. Fluorescent images: magnification ×100, scale bar: 250 μm; TEM magnification ×120000, scale bar: 200 μm. Results expressed as mean ± SD. value < 0.05; value < 0.01.