Equine Metabolic Syndrome Affects Viability, Senescence, and Stress Factors of Equine Adipose-Derived Mesenchymal Stromal Stem Cells: New Insight into EqASCs Isolated from EMS Horses in the Context of Their Aging
Table 2
Sequences of primers used in qPCR.
Gene
Primer
Sequence 5′-3′
Amplicon length
Accession number
Annealing temperature
GAPDH
F:
GATGCCCCAATGTTTGTGA
250
NM_001163856.1
61,7°C
R:
AAGCAGGGATGATGTTCTGG
p53
F:
TACTCCCCTGCCCTCAACAA
252
U37120.1
57,6°C
R:
AGGAATCAGGGCCTTGAGGA
p21
F:
GAAGAGAAACCCCCAGCTCC
241
XM_003365840.2
52,1°C
R:
TGACTGCATCAAACCCCACA
BAX
F:
TTCCGACGGCAACTTCAACT
150
XM_005607505.1
60,5°C
R:
GGTGACCCAAAGTCGGAGAG
CAS-9
F:
TCCTACTCCACCTTCCCAGG
150
XM_005607505.1
60,8°C
R:
CTCCGAAACAGCGTGAGCTA
BCL-2
F:
TTCTTTGAGTTCGGTGGGGT
164
XM_001490436.2
65,0°C
R:
GGGCCGTACAGTTCCACAA
BMP-2
F:
CGTCCTGAGCGAGTTCGAGT
249
XM_001493895.4
60,0°C
R:
CGCCGGGTTGTTTTTCCACT
RUNX-2
F:
CCAAGTGGCAAGGTTCAACG
165
XM_005603968.1
60,2°C
R:
TGTCTGTGCCTTCTGGGTTC
COLL-1
F:
GAAACTATCAATGGTGGTACCAAGT
265
XM_008524258.1
55,0°C
R:
AGCAGCCATCTACAAGAACAGT
COMP
F:
AGTGTCGCAAGGATAACTGCGTGA
238
NM_001081856.1
62,6°C
R:
TCCTGATCTGTGTCCTTCTGGTCA
COLL-2
F:
ATTCCTGGAGCCAAAGGATCTGCT
148
XM_005611082.1
62,7°C
R:
TGAAGCCAGCAATACCAGGTTCAC
VIM
F:
GCAGGATTTCTCTGCCTCTT
203
NM_001243145.1
55,0°C
R:
TATTGCTGCACCAAGTGTGT
Sequences, amplicon length, and annealing temperature of the primer sets. GAPDH: glyceraldehyde 3-phosphate dehydrogenase; p53: tumor suppressor p53; p21: cyclin-dependent kinase inhibitor 1A; BAX: BCL-2-associated X protein; BCL-2: B-cell lymphoma 2; CAS-9: caspase-9; BMP-2: bone morphogenetic protein 2; RUNX-2: runt-related transcription factor 2; COLL-1: collagen type I; COMP: cartilage oligomeric matrix protein; COLL-2: collagen type II; VIM: vimentin.