Research Article

Equine Metabolic Syndrome Affects Viability, Senescence, and Stress Factors of Equine Adipose-Derived Mesenchymal Stromal Stem Cells: New Insight into EqASCs Isolated from EMS Horses in the Context of Their Aging

Table 2

Sequences of primers used in qPCR.

GenePrimerSequence 5′-3′Amplicon lengthAccession numberAnnealing temperature

GAPDHF:GATGCCCCAATGTTTGTGA250NM_001163856.161,7°C
R:AAGCAGGGATGATGTTCTGG

p53F:TACTCCCCTGCCCTCAACAA252U37120.157,6°C
R:AGGAATCAGGGCCTTGAGGA

p21F:GAAGAGAAACCCCCAGCTCC241XM_003365840.252,1°C
R:TGACTGCATCAAACCCCACA

BAXF:TTCCGACGGCAACTTCAACT150XM_005607505.160,5°C
R:GGTGACCCAAAGTCGGAGAG

CAS-9F:TCCTACTCCACCTTCCCAGG150XM_005607505.160,8°C
R:CTCCGAAACAGCGTGAGCTA

BCL-2F:TTCTTTGAGTTCGGTGGGGT164XM_001490436.265,0°C
R:GGGCCGTACAGTTCCACAA

BMP-2F:CGTCCTGAGCGAGTTCGAGT249XM_001493895.460,0°C
R:CGCCGGGTTGTTTTTCCACT

RUNX-2F:CCAAGTGGCAAGGTTCAACG165XM_005603968.160,2°C
R:TGTCTGTGCCTTCTGGGTTC

COLL-1F:GAAACTATCAATGGTGGTACCAAGT265XM_008524258.155,0°C
R:AGCAGCCATCTACAAGAACAGT

COMPF:AGTGTCGCAAGGATAACTGCGTGA238NM_001081856.162,6°C
R:TCCTGATCTGTGTCCTTCTGGTCA

COLL-2F:ATTCCTGGAGCCAAAGGATCTGCT148XM_005611082.162,7°C
R:TGAAGCCAGCAATACCAGGTTCAC

VIMF:GCAGGATTTCTCTGCCTCTT203NM_001243145.155,0°C
R:TATTGCTGCACCAAGTGTGT

Sequences, amplicon length, and annealing temperature of the primer sets. GAPDH: glyceraldehyde 3-phosphate dehydrogenase; p53: tumor suppressor p53; p21: cyclin-dependent kinase inhibitor 1A; BAX: BCL-2-associated X protein; BCL-2: B-cell lymphoma 2; CAS-9: caspase-9; BMP-2: bone morphogenetic protein 2; RUNX-2: runt-related transcription factor 2; COLL-1: collagen type I; COMP: cartilage oligomeric matrix protein; COLL-2: collagen type II; VIM: vimentin.