The Combination of α-Tocopheryl Succinate and Sodium Selenite on Breast Cancer: A Merit or a Demerit?

Figure 4

Effect of 60 μM α-TOS, 2 μM SSe, and their combination on Bcl-2 protein levels after (a) 16 and (b) 48 hours, on Mcl-1 protein levels after (c) 16 and (d) 48 hours, on (e) caspase 9 and (h) caspase 7 activation after 16 and 48 hours using western blot technique, and on enzymatic activity of the caspase 3 class of proteases in cell lysate of MCF7 cells after (f) 16 and (g) 48 hours of exposure using ELISA technique. Western blot results were expressed as means ± SD of 3 independent experiments (). ELISA results are expressed as means ± SD of 2 replicate experiments (). Statistical significance of results was analyzed by one-way ANOVA using Tukey’s multiple comparison test. “A” significantly different from the respective control at ; “B” significantly different from respective α-TOS at ; “C” significantly different from respective SSe at .

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