Thioredoxin Binding Protein-2 Regulates Autophagy of Human Lens Epithelial Cells under Oxidative Stress via Inhibition of Akt Phosphorylation

Figure 6

TBP-2 mostly regulates autophagy in the initiation stage. (a) Vector cells and TBP-2 cells were treated with H₂O₂ (50 μM) for 12 hours, and wortmannin (10 μM) was added in the last 6 hours. The addition of wortmannin treatment decreased the phosphorylation of Akt at Ser473. The expression level of LC3-II in both types of cells decreased. (b) When bafilomycin A1 was added in the last 4 hours of the same H₂O₂ treatment, TBP-2 cells showed significantly higher level of LC3-II than NC cells (). (c) mCherry-LC3 puncta assay of the previous treatments showed similarly small number of LC3 dots in both cells when treated with 3-MA, and TBP-2 cells had significantly larger number of LC3 dots than NC cells when treated with bafilomycin A1 (). Data were presented as mean ± SEM of three independent experiments. . Scale bar, 25 nm.

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