Polydatin Attenuates H₂O₂-Induced Oxidative Stress via PKC Pathway

Figure 1

Effects of PD on the viability, adhesive ability, and migratory ability of OS-injured HUVECs. (a) HUVECs were subjected to H₂O₂ (400 μM) in the absence or presence of PD of different concentrations for 4 hours. The cell viability of HUVECs was measured by the MTT assay. (b) The adhesive ability of the HUVECs was assessed by performing an adhesion assay, and cell adhesion was expressed as an adhesion ratio. The number of adherent cells in the control group was set at 100%. The cell morphology was observed using inverted/phase-contrast microscopy, and images were obtained. (c) The migratory ability of the HUVECs was assessed by performing a wound healing assay, and the migratory ability was expressed as the mean distance between the two ends of the scratch. The mean distance in the control group was set at 100%. The results are expressed as mean ± SEM, = 3 (, compared to the control group, , , compared to the H₂O₂ group). Significant differences between groups were analyzed with one-way ANOVA.

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