Pharmacological Inhibition of NLRP3 Inflammasome Attenuates Myocardial Ischemia/Reperfusion Injury by Activation of RISK and Mitochondrial Pathways

Figure 2

Inflammasome expression and activation in the rat heart exposed to 30 min ischemia plus 20 or 60 min reperfusion, pretreated or not with INF4E. Representative Western blotting showing cardiac levels of NLRP3 and of downstream activation of caspase-1, GSDMDC1, and IL-1β cleavage assessed on heart extracts. Histograms report densitometric analysis normalized for the corresponding tubulin content. Data are means of 6 rats ± SEM. , , and versus Sham; , versus IR.

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