miR-21 Reduces Hydrogen Peroxide-Induced Apoptosis in c-kit⁺ Cardiac Stem Cells In Vitro through PTEN/PI3K/Akt Signaling

Figure 4

miR-21’s effect on PTEN expression in CSCs under normal condition. Cultured CSCs were treated with miR-21 mimics or its negative control scramble for 48 h, and then cells were harvested and subjected to RT-PCR or western blot. PTEN mRNA of control, scramble treated, or miR-21 mimics treated cells showed no significant difference (a), but PTEN protein dramatically decreased after miR-21 mimics treatment (b). compared with the other two groups. in each group.

(a)

(b)