The Cellular Response to Oxidatively Induced DNA Damage and Polymorphism of Some DNA Repair Genes Associated with Clinicopathological Features of Bladder Cancer

Table 2

Characteristics of allelic variants and some conditions for their detection.

Gene polymorphisms	Primer sequences	Restriction enzyme	PCR products (bp)
Nucleotide excision repair
ERCC2/XPD Asp312Asn rs1799793	(F) 5′-CTG TTG GTG GGT GCC CGT ATC TGT TGG TCT-3′; (R) 5′-TAA TAT CGG GGC TCA CCC TGC AGC ACT TCC T-3′	StyI	Asp/Asp: 507 + 244; Asp/Asn:  507 + 474 + 244 + 33; Asn/Asn: 474 + 244 + 33
ERCC2/XPD Lys751Gln rs13181	(F) 5′-GCC CGC TCT GGA TTA TAC G-3′; (R) 5′-CTA TCA TCT CCT GGC CCC C-3′	Pst I	Lys/Lys: 290 + 146; Lys/Gln: 290 + 127 + 146; Gln/Gln: 227 + 146
ERCC6/CSB Met1097Val rs2228526	(F) 5′-CCT GCT T CT AAC ATA TCT GT-3′; (R) 5′-AAT CAC TGA CAA CTC TTC TG-3′	Nla III	Met/Met: 123 + 78; Met/Val: 201 + 123 + 78; Val/Val: 201
ERCC6/CSB Gly399Asp rs2228528	(F) 5′-TGA AGA GTC TGA GTA TTT CC-3′; (R) 5′-ATC TTC ATC TCC ATC ATC TC-3′	RsaI	Gly/Gly: 180 + 91; Gly/Asp: 271 + 180 + 91; Asp/Asp: 271
Base excision repair
XRCC1; Arg399Gln; rs25487	(F) 5′-GGA CTG TCA CCG CAT GCG TCG G-3′; (R) 5′-GGC TGG GAC CAC CTG TGT T-3′	MspI	Arg/Arg: 115 + 34; Arg/Gln: 149 + 115 + 34; Gln/Gln: 149
OGG1; Ser326Cys; rs1052133	(F) 5′-CTG TTC AGT GCC GAC CTG CGC CGA-3′; (R) 5′-ATC TTG TTG TGC AAA CTG AC-3′	MboI	Ser/Ser: 224 + 23; Ser/Cys: 247 + 224 + 23; Cys/Cys: 247