Conservation of the Nrf2-Mediated Gene Regulation of Proteasome Subunits and Glucose Metabolism in Zebrafish

Figure 1

The expression of the proteasome subunit genes. (a) The gene expression of the indicated proteasome subunits in 8 h postfertilization (hpf) wild-type embryos injected with or without 100 pg of nrf2a mRNA at the 1-cell stage was analyzed by a real-time qPCR. Total RNA was extracted from 30 embryos for each sample. The expression of each gene was normalized to that of ef1α (means ± SEM), and the value in uninjected control was set to 1. Asterisks denote statistical significance (Control versus nrf2a overexpression, ; Student’s -test, for each group). (b) and (c) The gene expression of the indicated proteasome subunits in 4 dpf wild-type or nrf2a^fh318/fh318 mutant larvae that were treated (or not treated) with 100 μM DEM for 6 h (b) and 12 h (c) was analyzed by a real-time qPCR. The expression of each gene was normalized to that of ef1α (means ± SEM), and the value in untreated wild-type control was set to 1.

(a)

(b)

(c)