Immediate Remote Ischemic Postconditioning Reduces Brain Nitrotyrosine Formation in a Piglet Asphyxia Model

Figure 4

Colocalization of nitrotyrosine deposits and oxidative stress/neural markers. Nitrotyrosine deposits were mostly visible in neurons of HI animals (white arrows, (a)), with some colocalization also present in RIPostC-treated piglets (b). This colocalization was also observed to a much smaller extent in microglia of RIPostC animals (c-d), endothelial cells in the HI group (g-h), and toxic nNOS (i-j) and iNOS (k-l) for both treatments but not in astroglial (e-f). iNOS double labelling (black box) showed its expression in neurons (m-n) and astrocytes (q-r) of both HI and RIPostC animals but not in endothelial cells (s-t). iNOS was also expressed by microglia in the RIPostC group (o-p).