Identification of Redox and Glucose-Dependent Txnip Protein Interactions

Figure 1

BioID system for identification of Txnip interacting proteins. Myc- was fused to the aminoterminus of Txnip (-Txnip) to screen for protein interactions. catalyzes a two-step reaction: first, generation of reactive biotinyl-AMP from biotin and ATP, and second, the attachment of that biotinyl-AMP to a specific lysine on an interacting/proximal protein. Streptavidin beads are used to affinity-purify biotinylated proteins, which then are analyzed by mass spectrometry.