Treatment with morin reduced inflammatory cells infiltration, goblet cell hyperplasia, collagen deposition, and the expression of MMP-9 in lung tissue (magnification 400x). (a) Cell numbers and differentiation in BALF were determined by hemocytometer, and at least 200 cells were counted ( per group). (b) Lung sections were stained with H&E to analyze the infiltration of inflammatory cells, PAS to assess goblet cell hyperplasia, Masson’s trichrome to evaluate the subepithelial deposition of collagen and fibrosis, and immunohistochemistry to assess the distribution of MMP-9. Scale bar: 50 μm. (c) The layers of inflammation cells were counted and the total inflammation score was summed up with peribronchial (PB) and perivascular (PV) inflammation scores. (d) PAS-positive and PAS-negative epithelial cells were counted, and the percentage of PAS-positive cells per bronchiole was calculated. (e) Masson’s trichrome staining analysis of collagen deposition was calculated. (f) The MMP-9 expression was evaluated and the total MMP-9 staining score was multiplied up with percentage of positive epithelial cells and staining intensity scores. Values represented as mean ± SD ( per group). compared with the control group, and compared with the OVA group.
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