Figure 7: Effect of D-limonene on the expression of P-p38 in HLECs in response to H2O2. The cells were incubated with 1000 μM D-limonene for 24 h with/without pretreatment with 10 μM SB203580 for 30 min before 100 μM H2O2 incubation for 1 h. (a) Phosphorylation of protein was measured by western blot using phospho-p38 MAPK antibody (lane 1). Total p38 MAPK protein was also measured using anti-p38 MAPK antibody (lane 2). Representative blots for three independent experiments are shown. (b) Quantitative analysis was performed by measuring intensity relative to the untreated control. .