Band 3 Erythrocyte Membrane Protein Acts as Redox Stress Sensor Leading to Its Phosphorylation by p⁷² Syk

Figure 2

Protein phosphorylation analysis by mass spectrometry. Tyrosine phosphorylated proteins after 30-minute incubation of RBCs with 0.5 mM diamide (a) and serine phosphorylated proteins after 2-hour incubation of RBCs with 1 mM PHZ (b). Phosphorylated proteins were analyzed by mass spectrometry (MALDI-TOF) (Table 1). Band numbering in panels (a) and (b) identifies the proteins listed in Table 1.

(a)

(b)