Critical Role of FoxO1 in Granulosa Cell Apoptosis Caused by Oxidative Stress and Protective Effects of Grape Seed Procyanidin B2

Figure 1

Effects of diquat alone or combined with GSPB2 on ROS levels in granulose cells. Mice were treated as described in Section 2 and granulosa cells were isolated for ROS detection. (a) The ROS levels were detected by dihydroethidium bromide fluorescence (red), and nuclei were counterstained with DAPI (blue). (b) Quantification of ROS levels in granulose cells. Results are expressed as means ± SEM, , versus control, and versus diquat alone.

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