Critical Role of FoxO1 in Granulosa Cell Apoptosis Caused by Oxidative Stress and Protective Effects of Grape Seed Procyanidin B2

Figure 2

Effects of diquat alone or combined with GSPB2 on granulosa cell apoptosis in antral follicle. Mice were treated as described in Section 2 and the right ovaries were embedded in paraffin and serially sectioned for TUNEL assay. (a) Follicular granulosa cell apoptosis was assayed by TUNEL. (b) Quantification of TUNEL-positive follicles. (c) The relative expression levels of apoptosis-related genes. (d) The relative expression level of FoxO1 in granulosa cells. Results are expressed as means ± SEM, , versus control, and versus diquat alone.

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