Critical Role of FoxO1 in Granulosa Cell Apoptosis Caused by Oxidative Stress and Protective Effects of Grape Seed Procyanidin B2

Figure 8

GSPB2 prevented FoxO1 expression and nuclear localization. Cultured cells were pretreated with GSPB2 (10 μmol/L) for 24 h and exposed to H₂O₂ (150 μM) for 6 h. (a) Quantitative RT-PCR was employed to detect the relative expression of FoxO1. (b) Western blotting analysis of FoxO1 protein levels in cultured granulosa cells treated with or without GSPB2. (c) Quantification of the relative FoxO1 protein level with densitometry. (d) Subcellular localization of FoxO1 in response to oxidative stress. Scale bars are 100 μm. (e) The fluorescent relative quantitative analysis of nuclear/cytoplasm FoxO1. Data was shown as mean ± SEM, . , H₂O₂ alone group versus H₂O₂-free group (control); versus H₂O₂ alone group.

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