Table of Contents Author Guidelines Submit a Manuscript
Oxidative Medicine and Cellular Longevity
Volume 2016, Article ID 6354261, 9 pages
http://dx.doi.org/10.1155/2016/6354261
Research Article

Skin Aging-Dependent Activation of the PI3K Signaling Pathway via Downregulation of PTEN Increases Intracellular ROS in Human Dermal Fibroblasts

1Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan 570-749, Republic of Korea
2Division of Hematology/Oncology, Gachon University Gil Medical Center, Incheon 405-760, Republic of Korea
3Department of Microbiology & Immunology, Institute of Medical Science, Chonbuk National University Medical School, Jeonju 560-182, Republic of Korea
4Department of Biochemistry, Institute of Medical Science, Chonbuk National University Medical School, Jeonju 560-182, Republic of Korea
5Department of Pediatrics, Institute of Clinical Science, Chonbuk National University Medical School, Jeonju 560-182, Republic of Korea
6Department of Korean Physiology, Wonkwang University School of Korean Medicine, Iksan 570-749, Republic of Korea
7Department of Oral Biochemistry and Institute of Biomaterials, Implant, School of Dentistry, Wonkwang University, Iksan 570-749, Republic of Korea

Received 25 March 2016; Revised 4 July 2016; Accepted 26 July 2016

Academic Editor: Juan F. Santibanez

Copyright © 2016 Eun-Mi Noh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary figure 1: NADPH oxidase activity increases in aged HDFs.

Supplementary figure 2: The expression of PIP3 increases with increasing PD of HDFs.

Supplementary figure 3: Increased PIP3 levels inhibited through suppression of PI3K in aged HDFs.

Supplementary figure 4: Inactivation of PTEN increases PIP3 expression.

Supplementary figure 5: Expression of NOXs in young and aged HDFs.

Supplementary figure 6: Elevation of NADPH oxidase activity decreased by PKCζ inhibition in aged HDFs.

  1. Supplementary Material