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Oxidative Medicine and Cellular Longevity
Volume 2016 (2016), Article ID 6439037, 13 pages
http://dx.doi.org/10.1155/2016/6439037
Research Article

Oxidative Stress Assessment in Response to Ultraendurance Exercise: Thiols Redox Status and ROS Production according to Duration of a Competitive Race

1Institute of Bioimaging and Molecular Physiology, National Research Council (CNR), Via Fratelli Cervi 93, 20090 Segrate, Italy
2Institute of Clinical Physiology, National Research Council (CNR), Niguarda Ca’ Granda Hospital, Via G. Moruzzi 1, 56124 Pisa, Italy
3Telematic University San Raffaele, Via F. Daverio 7, 20122 Milan, Italy

Received 21 April 2016; Revised 23 May 2016; Accepted 24 May 2016

Academic Editor: Steven McAnulty

Copyright © 2016 Alessandra Vezzoli et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. Response to an ultraendurance competitive race on thiols redox status, reactive oxygen species (ROS) production, and oxidative stress (OxS) was investigated according to duration. Methods. Twenty-four elite runners were examined: six completed 50 km and eighteen 100 km. Blood and urine samples were collected before and immediately after the race. Erythrocytes and plasma aminothiols by high-performance liquid chromatography, total antioxidant capacity (TAC), and OxS biomarkers (protein carbonyl (PC), thiobarbituric acid-reactive substances (TBARS), 8-isoprostane (8-iso-PGF2α), and 8-OH-2-deoxyguanosine (8-OH-dG)) by immunoenzymatic assays and ROS production by Electron Paramagnetic Resonance were assessed. Results. Significant increases ( between <0.05 and <0.0001) were recorded in plasma total and oxidized aminothiols concentration and TAC () only after 100 km: plasmatic (ROS production (+12 versus +29%), PC (+54 versus +115%), and TBARS (+28 versus +55%)) and urinary (8-OH-dG.creatinine−1 (+71 versus +158%) and 8-iso-PGF2α.creatinine−1 (+43 versus +135%)) concentrations for 50 and 100 km (duration 4 h 3′ versus 8 h 42′), respectively. Conclusion. Very prolonged ultraendurance exercise causes an increase in ROS production and OxS depending on specific biomarker examined but always linearly and directly related to exercise duration. Redox status of erythrocytes was preserved. A relationship between running performance and both prerace ROS production and antioxidant-redox status was found in 100 km race.