The H2S Donor NaHS Changes the Expression Pattern of H2S-Producing Enzymes after Myocardial Infarction
Endogenous H2S protected ischaemic myocardium from myocardial infarction injury. (a) Characterization of CSE knock-out mice (CSE KO). Left panel: PCR analysis of genomic DNA from CSE KO mice and wide type mice (WT). Middle panel: real-time PCR analysis of CSE mRNA expression in the hearts from WT and CSE KO mice; right panel: representative western blot of CSE protein expression. (b) Plasma H2S levels in WT and CSE KO mice with (MI) or without (sham) surgery. Plasma H2S levels from the highest to lowest were as follows: WT without surgery, CSE KO without surgery, WT with MI surgery, and CSE KO with MI surgery; (c) infarct area in CSE KO group was larger than that in WT group detected with Evans’ Blue-TTC staining; (d) expression of CBS and 3-MST increased in CSE KO mice. Numbers inside bars denote the number of animals per group. Values are mean ± SE. and .