Zinc Chelation Mediates the Lysosomal Disruption without Intracellular ROS Generation

Figure 4

(a) Histogram of lysosomal disruption assay with Acridine Orange measured by flow cytometry. Black line shows control population and red line shows increase in the percentage of cells exhibiting a weaker fluorescence (subpopulation). (b) Subpopulation (weaker fluorescence) of 670 nm fluorescence analysis of SH-SY5Y after exposure to 5 or 25 μM TPEN for 4, 12, and 24 hours. (c) SH-SY5Y cells were pretreated with 300 μM antipain dihydrochloride (A.D.: cathepsins inhibitor) for 1 hour and subsequently incubated with 25 μM TPEN for 12, 24, and 48 hours and analyzed by MTT assay. Untreated cells were used as control and treated cells with 25 μM TPEN were used as positive control of cell death. Data represent the mean values ± standard deviation (), and significant differences between treated and untreated cells were , , and ; and significant differences between treated and 25 μM TPEN treated cells were and .

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