Zinc Chelation Mediates the Lysosomal Disruption without Intracellular ROS Generation

Figure 5

Western blot indicates whether expression levels of apoptotic proteins were upregulated or not during TPEN therapy with zinc depletion in neuroblastoma cells. SH-SY5Y cells were treated with 25 μM TPEN. At each time point, 100 μg of total proteins from the total cell lysates was loaded onto each lane for the detection of p38 (a and c) and caspase 3 (b and c). (c) The p38 MAPK and caspase 3 levels were determined after inhibition of the p38 protein with SB202190. (d) SH-SY5Y cells were pretreated with 300 μM antipain dihydrochloride (A.D.: cathepsins inhibitor) and/or 10 μM SB202190 for 1 hour and subsequently incubated with 25 μM TPEN for 12, 24, and 48 hours. Untreated cells were used as control and cells treated with 25 μM TPEN were used as positive control of cell death in these cases. The Western blot images represent three independent experiments, and significant differences between treated and untreated cells were and ; and significant differences between treated and 25 μM TPEN treated cells were and .

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